How do you use a Dengue IgG/IgM test?

Table of Contents

1. Introduction
2. Dengue IgG/IgM Test Overview
3. Methodology of Dengue IgG/IgM Testing
4. Interpretation of Results
5. QL Biotech Company Solutions
6. References

Introduction

Dengue fever remains a significant public health concern in tropical and subtropical regions. Rapid and accurate diagnosis is crucial for effective management and control of outbreaks. The Dengue IgG/IgM test is a widely used diagnostic tool that helps in identifying both primary and secondary infections.

Dengue IgG/IgM Test Overview

The Dengue IgG/IgM test is a serological assay that detects antibodies specific to the Dengue virus in human blood samples. This test provides crucial information for determining the phase of the infection, distinguishing between acute and past infections.

Methodology of Dengue IgG/IgM Testing

1. Sample Collection:

   Blood samples are typically collected via venipuncture. Serum or plasma is separated from the cellular components for testing.

2. Assay Procedure:

   The test is commonly conducted using an ELISA (Enzyme-Linked Immunosorbent Assay) method. The test kit contains antigens from the Dengue virus, which interact with the patient's antibodies.

3. Sensitivity and Specificity:

   The IgG component usually detects past infections with more than 90% sensitivity, whereas IgM, with a sensitivity of approximately 85%, is indicative of recent infections.

Interpretation of Results

Results from the Dengue IgG/IgM test must be interpreted with caution, considering cross-reactivity with other flaviviruses. A positive IgM result typically indicates a current or recent infection, whereas a positive IgG result suggests a past infection.

1. IgM Positive:

   Indicates a current or recent Dengue infection. It is important for early clinical intervention.

2. IgG Positive:

   Suggests a past infection and potential immunity to certain serotypes of the virus.

3. Both IgG and IgM Positive:

   Potential secondary Dengue infection, which may lead to more severe clinical manifestations.

QL Biotech Company Solutions

QL Biotech offers state-of-the-art solutions for Dengue IgG/IgM testing that include highly sensitive and specific ELISA kits. Their products are designed to deliver rapid results with minimized false positive rates, improving the reliability of diagnosis.

1. Enhanced Specificity:

   QL Biotech uses advanced antigen formulations to reduce cross-reactivity, ensuring more accurate IgG and IgM detection.

2. Rapid Turnaround:

   Results can be obtained within 2 hours, facilitating quick clinical decision-making.

References

1. World Health Organization. Dengue and severe dengue. [Link]
2. Centers for Disease Control and Prevention. Dengue Diagnostic Testing. [Link]
3. QL Biotech Company. Dengue IgG/IgM ELISA Kits. [Product Brochure]